phospho slp 76 ser376 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc phospho slp 76 ser376
Phospho Slp 76 Ser376, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+slp+76+ser376/Phospho-SLP-76+(Ser376)+Rabbit+mAb/pm41611868-611-53-84
Average 94 stars, based on 20 article reviews

phospho slp 76 ser376 - by Bioz Stars, 2026-09

94/100 stars

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Western Blot:

Article Title: SIRT2-mediated deacetylation of LCK governs the magnitude of T cell receptor signaling
Article Snippet: Image acquisition was performed with the Amersham imager 600 system (GE Healthcare Bio-Sciences). .. Immunoblotting was performed using primary Abs against acetyl-lysine (9441, 1:1,000 dilution), SIRT2 (12650, 1:1,000 dilution), LCK (2752, 1:1,000 dilution), ZAP70 (3165, 1:1,000 dilution) LAT (45533, 1:1,000 dilution) SLP-76 (25361, 1:1,000 dilution), PLCγ1 (5690, 1:1,000 dilution), Erk1/2 (9102, 1:1,000 dilution), GFP (2956, 1:1,000 dilution), Phospho-LAT (Tyr220) (20172, 1:1,000 dilution), Phospho-PLCγ1 (Tyr783) (14008, 1:1,000 dilution), Phospho-SLP-76 (Ser376) (14745, 1:1,000 dilution), Phospho-ZAP70 (Tyr319)/Syk (Tyr352) (2717, 1:1,000 dilution), Phospho-Src Family (Tyr416) (6943, 1:1,000 dilution), phospho-LCK (Tyr505) (37458, 1:1,000 dilution) and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370, 1:1,000 dilution) from Cell Signaling, β-actin (ab8227, 1:5,000 dilution) and GAPDH (ab181602, 1:10,000 dilution) from Abcam. .. Human histidine-LCK fusion protein was manufactured, immobilized on HisPur cobalt resin (Thermo Fisher Scientific) and used as a bait protein.

Article Title: SIRT2-mediated deacetylation of LCK governs the magnitude of T cell receptor signaling.
Article Snippet: Image acquisition was performed with the Amersham imager 600 system (GE Healthcare Bio-Sciences). .. Immunoblotting was performed using primary Abs against acetyl-lysine (9441, 1:1,000 dilution), SIRT2 (12650, 1:1,000 dilution), LCK (2752, 1:1,000 dilution), ZAP70 (3165, 1:1,000 dilution) LAT (45533, 1:1,000 dilution) SLP-76 (25361, 1:1,000 dilution), PLCγ1 (5690, 1:1,000 dilution), Erk1/2 (9102, 1:1,000 dilution), GFP (2956, 1:1,000 dilution), Phospho-LAT (Tyr220) (20172, 1:1,000 dilution), Phospho-PLCγ1 (Tyr783) (14008, 1:1,000 dilution), Phospho-SLP-76 (Ser376) (14745, 1:1,000 dilution), Phospho-ZAP70 (Tyr319)/Syk (Tyr352) (2717, 1:1,000 dilution), Phospho-Src Family (Tyr416) (6943, 1:1,000 dilution), phospho-LCK (Tyr505) (37458, 1:1,000 dilution) and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370, 1:1,000 dilution) from Cell Signaling, β-actin (ab8227, 1:5,000 dilution) and GAPDH (ab181602, 1:10,000 dilution) from Abcam. .. Pulldown polyHis protein–protein interaction assay Human histidine-LCK fusion protein was manufactured, immobilized on HisPur cobalt resin (Thermo Fisher Scientific) and used as a bait protein.

Incubation:

Article Title: Co-opting signalling molecules enables logic-gated control of CAR T cells.
Article Snippet: Although chimeric antigen receptor (CAR) T cells have altered the treatment landscape for B cell malignancies, the risk of on-target, off-tumour toxicity has hampered their development for solid tumours because most target antigens are shared with normal cells1,2.. Researchers have attempted to apply Boolean-logic gating to CAR T cells to prevent toxicity3–5; however, a truly safe and effective logic-gated CAR has remained elusive6.. Here we describe an approach to CAR engineering in which we replace traditional CD3ζ domains with intracellular proximal T cell signalling molecules.

Article Title: Co-opting signalling molecules enables logic-gated control of CAR T cells
Article Snippet: .. The following primary antibodies were used: phospho-PLCγ1 Ser1248 (D25A9, Cell Signaling), PLCγ1 (D9H10, Cell Signaling), phospho-SLP-76 Ser376 (D9D6E, Cell Signaling), SLP-76 (polyclonal, Cell Signaling), phospho-AKT Ser473 (Polyclonal, Cell Signaling), AKT (40D4, Cell Signaling), Phospho-ERK1/2 Thr202/Tyr204 (Polyclonal, Cell Signaling), ERK1/2 (Polyclonal, Cell Signaling), Phospho-c-JUN Ser73 (D47G9, Cell Signaling), Phospho-NF-κB Ser536 (93H1, Cell Signaling) and NF-κB (D14E12, Cell Signaling). scRNA-seq B7-H3-targeting CAR T cells were incubated with or without B7-H3 + Nalm6 for 6 h at a 1:1 E:T ratio, then stained for CD45 and CAR. .. The following primary antibodies were used: phospho-PLCγ1 Ser1248 (D25A9, Cell Signaling), PLCγ1 (D9H10, Cell Signaling), phospho-SLP-76 Ser376 (D9D6E, Cell Signaling), SLP-76 (polyclonal, Cell Signaling), phospho-AKT Ser473 (Polyclonal, Cell Signaling), AKT (40D4, Cell Signaling), Phospho-ERK1/2 Thr202/Tyr204 (Polyclonal, Cell Signaling), ERK1/2 (Polyclonal, Cell Signaling), Phospho-c-JUN Ser73 (D47G9, Cell Signaling), Phospho-NF-κB Ser536 (93H1, Cell Signaling) and NF-κB (D14E12, Cell Signaling). scRNA-seq B7-H3-targeting CAR T cells were incubated with or without B7-H3 + Nalm6 for 6 h at a 1:1 E:T ratio, then stained for CD45 and CAR.

Staining:

other:

Article Title: Deubiquitinase BAP1 is crucial for surface expression of T cell receptor (TCR) complex, T cell-B cell conjugate formation, and T cell activation.
Article Snippet: Primary antibodies used at indicated dilutions included BAP1 (1:1000, #13271), GAPDH (1:1000, #97166S), Phospho-p38 MAPK (Thr180/Tyr182) (1:1000, #4511), p38 MAPK (1:1000, #8690), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (1:1000, #4370), ow nloaded from https://academ ic.oup.com /jleukbio/advance-article/doi/10.1093/jleuko/qiae184/7742416 by guest on 25 N ovem ber 2024 p44/42 MAPK (Erk1/2) (1:1000, #4695), Phospho-IκBα (Ser32) (1:1000, #2859), IκBα (1:1000, #9242), β-Actin (1:1000, #3700), Phospho-SLP-76 (Ser376) (E3G9U) (1:1000 #76384), SLP-76 (1:1000 #4958), Phospho-LAT (Tyr220) (1:1000, #3584), PhosphoPLCγ1(Tyr783)(D6M9S) (1:1000, #14008), PLCγ1 (D9H10) (1:1000, #5690) and were sourced from Cell Signaling Technology.

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